The AutoXT Total gDNA Kit is an automated solution for rapid, high-yield extraction of high-purity genomic DNA (gDNA). It uses the magnetic bead method within an automated device and features an optimized buffer system and excellent lysis efficiency. The kit is highly versatile, capable of processing gDNA from diverse samples like whole blood, tissue, buffy coat, bacteria, etc. The resulting high-purity DNA is ideal for advanced genomic analysis, including Next-Generation Sequencing (NGS).
Research/Application area
- Pathogen Detection analysis
- PCR & Real-time PCR
- Genotyping
- Infectious Disease Research
AutoXT Total gDNA kit
Individual Type (48T) Well-plate Type (96T) 48 Prefilled cartridges 6 Prefilled Well plates 6 Plunger tips 12 Plunger tips Proteinase K, RNase A
(Lyophilized)
Proteinase K, RNase A
(Lyophilized)
Cartridge Rack
(Individual Type only)
Q: DNA extraction yield is lower than expected. What should I do?
A: It is believed that the sample was not properly homogenized. Check the extraction protocol settings of the Miracle-AutoXT Nucleic Acid Extraction System equipment to ensure that they are appropriately considered for the extraction target. Before using the equipment, check the fixation status of the cartridge to the rack and the set temperature of the block (appropriate temperature setting is required) before proceeding.
Q: Contamination of the extracted nucleic acid is suspected.
A: When adding lysate to the product's cartridge, be careful not to splash it. To minimize contamination of adjacent cartridges, it is recommended that samples be separated from the rack when applying them. To prevent contamination between samples, use a new Plunger Tip for each sample.
Q: I would like to know the yield of the extracted sample. Is there an effective method other than absorbance measurement?
A: This product extracts the entire genomic DNA present in the sample. Absorbance measurement alone has a wide range of target accuracy and error in small quantities. For more accurate judgment, we recommend a method of quantification by performing PCR with a quantitative function, such as real-time PCR.
