Q: We want to extract RNA from a plant using easy-BLUE. The protocol says that a P-buffer is needed. What is the composition of a P-buffer?
A: P-Buffer (for Plant): Final 0.8M sodium citrate 1.2M NaCl. The information can also be found in the 'PREPARING SOLUTION BEFORE USE' section of the product manual. It is also available exclusively for the "easy-spin™ llp Plant RNA Extraction Kit" with the addition of a column for plant RNA extraction.
Q: I would like to extract RNA from the Kidney. I am afraid that RNA degradation might have happened while performing homogenization because kidney tissue is very sticky. Is there any other possible way to increase the recovery rate of RNA?
A: First of all, try to add liquid N2 after obtaining a thin tissue fragment and then perform homogenization to increase yield and purity. Also, please be cautious of the repetition of freezing/thawing. Please use 10mg of the kidney sample because the tissue is very active.
Q: I see gDNA contamination often. Is there any possible way to minimize gDNA contamination?
A: While moving the aqueous layer, do not try to pipette hard and pipette only a volume of 400 μL. gDNA may mix with the interface layer when you try to extract large amounts of RNA.
Q: Do blue lights from easy-Blue affect the purity of RNA and the recovery rate?
A: The reason for blue light is because of the pH indicator, which increases the RNA recovery rate. Especially, it gives blue light, so it is convenient for separating the aqueous layer.
Q: I was using Easy-BLUE and I did not use it for a long time, but, blue color has changed to black. Can I keep using this kit? Does it affect RNA recovery rate?
A: Blue light from easy-BLUE is because of the pH indicator. The color change has happened because the pH has changed. Therefore, the RNA recovery rate may decrease, so we recommend that you use a new kit. Also, the reason for the color change is that the kit might have been stored at room temperature. Please store the kit at 4℃ to obtain a high RNA recovery rate.
