The FaSTAR-XT Tissue DNA Kit is designed for use with the FaSTARpre96 automatic nucleic acid extraction system, providing efficient purification of genomic DNA from a variety of tissue samples. The kit utilizes magnetic beads for rapid and effective nucleic acid binding, and a proprietary buffer system to achieve optimal elution.
Operation of the FaSTARpre96 system requires installation of the FaSTAR-XT firmware and the exclusive use of the FaSTARpre96 high-intensity magnetic rod and sleeve to ensure correct protocol execution.
The nucleic acid purification workflow is streamlined and requires minimal manual intervention prior to automated processing. The resulting eluted DNA is of high purity, making it ideally suited for downstream applications such as PCR and real-time PCR. The FaSTAR-XT Tissue DNA Kit is capable of processing between 1 and 96 samples in a single run.
FaSTAR-XT Tissue DNA Kit
Q: Lower nucleic acid recovery than expected?
A: There may be several possible causes. Below are the comments following the situation:
- Sample homogenization was incomplete.
- Incomplete homogenization results in loss of yield within particulates and clumps of debris.
- Refer to the sample preparation instructions in the manual and prepare the sample.
- The starting samples were compromised. Ensure samples were collected, shipped, and stored according to guidelines.
- The FaSTARprep96 was set for the wrong method. Ensure correct method selection (Tissue DNA/Food Safety/Halal Testing).
- Check the mixing sleeve installation and plate orientation.
- Verify the amount and storage conditions of starting materials.Q: Cross contamination?
A: Avoid splashing when adding solvent to the plate. To prevent contamination, each sample has a separate mixing sleeve and is designed to minimize exposure during rotary mixing.
