The G-DEX™ IIc Genomic DNA Extraction Kit is a versatile, solution-based product designed for the efficient extraction of high-yield, purified genomic DNA from a variety of sample types, including cells, tissues, and bacteria. Adaptable to different starting amounts, it utilizes an optimized buffer system to ensure consistent results, with yields such as 1 mg from 1x10⁸ cells or 1 g tissue, and 50 μg from 1 ml of bacteria. The kit delivers DNA of high purity (OD260:280 = 1.9–2.1) and reproducibility, making it well-suited for demanding genetic research applications.
Extraction is performed in just four simple steps—lysis, protein removal, DNA pellet acquisition, and DNA hydration—allowing completion within 20 to 60 minutes. Specialized buffers, including Cell Lysis Buffer and PPT Buffer for protein precipitation without heating, further streamline the process. The purified DNA is ready for downstream uses such as PCR, Southern blotting, and cDNA chip probe preparation. Overall, the kit offers convenience, reliability, and exceptional results for researchers seeking high-quality genomic DNA.
Research/Application Area
- PCR & Real-time PCR
- Southern blotting
- SNP
- Gene Cloning
- Genotyping
- Pathogen Research etc.
G-DEX™ IIc Genomic DNA Extraction Kit (for Cell/Tissue)
Contents Quantity for 300 Tests Lysis Buffer 60 ml x 1 ea PPT Buffer 25 ml x 1 ea DNA Rehydration Buffer 25 ml x 1 ea RNase A (Lyophilized powder) 3 mg x 1 tube Manual 1 ea Q: Is there any protocol for applying Tissue? The product protocol is only for the protocol for the cell.
A: When applying the assay, add 150 ~ 200ul of lysis buffer per 10mg and proceed with homogenization. After that, proceed as same as cell protocol.
Q: We are currently using P company for genetic testing of Hanwoo. Is it available as an alternative to this product?
A: Yes. It's possible to use. Our products consist of Cell lysis, Protein PPT, DNA Rehydration buffer, etc., and can be used without any change of protocol.
Q: I am doing cell culture on a 150mm culture dish. Is it possible to extract DNA all at once from a 150mm culture dish cells with this product?
A: Extraction is possible. Table1 summarizes the recommended buffer amount based on the initial cell number in this product protocol. For 150mm dish, about 2x107 cells (Confluency) is enough to start with 3ml of initial lysis buffer. Before starting, measure the number of cells before proceeding.
Q: The cell pellet floats without lysis after adding lysis buffer. I just went ahead and the yield was very low. What is the cause of this, and what is the solution?
A: Cell pellet is obtained by centrifugation of cells in PBS or PBS. When the lysis buffer is added directly to the cell pellet thus obtained, the cell clump will float without releasing, resulting in a loss of yield. A solution to this is to leave some media in the supernatant after cell pelleting. The amount of media left is not important because it is for resuspend of the cell pellet. Vortexing or tapping to completely resuspend the cell pellet. After confirming that there is no accumulated cell, add lysis buffer and perform the protocol afterwards.
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