The G-spin™ Genomic DNA Extraction Mini Kit (for Bacteria) stands out for its ability to efficiently isolate high-quality genomic DNA from both gram-positive and gram-negative bacteria. Unlike traditional manual extraction methods, which can be time-consuming and often result in low purity, this kit streamlines the process with a user-friendly spin column format.
A key feature is its specialized buffer system, which not only stabilizes bacterial genomic DNA but also allows for the extraction of large, intact DNA fragments without shearing. This ensures that the DNA is suitable for demanding downstream applications. The kit leverages advanced GxN technology to achieve both high purity and high yield, making it an optimal choice for researchers who require reliable results.
With a proven track record among researchers, the kit is ideal for a range of molecular biology applications, including PCR, Southern blot analysis, RAPD, and sequencing. Its consistent performance and ease of use make it a trusted tool in genomic DNA extraction.
G-spin™ Genomic DNA Extraction Kit (for Bacteria)
Q: I am trying to extract the genomic DNA of Bacteria, but I am not sure whether it is gram positive or negative. Are there any products available for the iNtRON?
A: We recommend the G-Spin ™ Genomic DNA Extraction Kit (for Bacteria) and refer to the Gram positive bacteria protocol.
Q:What is the role of Pre-buffer in order to use only Pre-buffer for Gram-positive bacteria protocol?
A:Gram positive bacteria have thicker peptidoglycan layers than negative bacteria. Through an enzyme such as Lysozyme, it is necessary to disassemble, and Pre-buffer can decompose the thick outer wall of Gram positive.
Q:Is there a way to increase the yield of genomic DNA?
A:When using this product, increase the lysis time to complete the lysis process, and reduce the elution volume to obtain a higher recovery rate.
Q:When I put EtOH in washing buffer, I did not have absolute EtOH, so I put 80% EtOH. Is it okay?
A:The use of Absolute EtOH ensures complete removal of the salt, maximizing the efficiency of the washing step.
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