top of page
i-genomic BYF DNA Extraction Mini Kit
The i-genomic™ BYF DNA Extraction Mini Kit is a professional-grade solution specifically engineered for the efficient isolation of high-yield, highly purified DNA from challenging biological samples, including Gram-positive bacteria, yeast, and fungi. This kit stands out for its reliability and reproducibility, offering four optimized protocols tailored for different sample types, with dedicated procedures for fungal tissue and yeast.
 
Its advanced buffering system, which utilizes chaotropic salts and detergents along with pre-lysis and lysis buffers, ensures effective cell disruption and maximizes DNA recovery. The inclusion of specialized buffers (Buffer MVP and Buffer MP) further enhances lysis efficiency for yeast samples.
 
Comprehensive performance data is provided for up to 23 sample types, and the kit comes with clear, user-friendly instructions to streamline the extraction process. These features collectively make the i-genomic™ BYF DNA Extraction Mini Kit a robust and reliable choice for researchers seeking high-quality DNA extraction from difficult-to-lyse organisms.
 
Research/Application area
  1. PCR & Real-time PCR
  2. Southern Blotting
  3. SNP
  4. Gene Cloning
  5. Genotyping
  6. Pathogen Research etc

i-genomic BYF DNA Extraction Mini Kit

  • Contents Quantity
    Buffer MYP (for Yeast Pre-Lysis Buffer) 12 ml x 1 ea
    Buffer MP (Pre-Lysis Buffer) 7 ml x 1 ea
    Buffer MG (Lysis Buffer) 15 ml x 1 ea
    Buffer MB (Binding) 15 ml x 1 ea
    Buffer MW (Concentration) 10 ml x 1 ea
    Buffer ME (Elution) 20 ml x 1 ea
    Spin Column (Blue color O-ring) 50 Columns
    Collection Tube 100 tube
    RNase A, lyophilized 6 mg x 1 tube
    Proteinase K, lyophilized 12 mg x 1 tube
    Lysozyme, lyophilized 30 mg x 1 tube
    Manual 1 ea

  • Q: Are there any products available for extracting DNA from Yeast?

    A: We recommend the i-genomic BYF DNA Extraction Mini Kit. This product can extract genomic DNA from yeast, but also from various types of BYF (B: Gram positive Bacteria, Y: Yeast, F: Fungi) samples. In order to obtain the optimal protocol, we tested 23 BYF samples and increased DNA recovery and purity. However, one thing to note is that when yeast extracts DNA from yeast, we recommend that you purchase lyticase or zymolase separately from this product.

    Q: What kinds of buffers are available in the product? What is the role of each buffer?

    A: Due to the nature of the applied sample, the outer wall of the cell is so thick that it undergoes a pre-lysis process, and various buffers are used to obtain high purity DNA. First, MYP buffer is Yeast Pre-Lysis Buffer, and the outer wall is very strong due to the characteristic of yeast. Therefore, when yeast sample is applied, the lysis process proceeds one step. For yeast samples, occasionally lyticase or zymolase can be used to increase the DNA recovery rate. MP buffer is a pre-lysis buffer used for samples other than yeast. Next, the MG buffer is the lysis buffer, and the MB buffer allows DNA binding to the column. You can think of MW buffer as column washing buffer and ME buffer as elution Buffer.

bottom of page