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i-genomic Plant DNA Extraction Mini Kit
The i-genomic™ Plant DNA Extraction Mini Kit is a spin-type solution designed for the rapid and reliable extraction of highly purified genomic DNA from a wide variety of plant specimens and processed foods. It offers five optimized protocols to maximize reliability and reproducibility for different sample types, including challenging plant materials with complex secondary metabolites.
 
The kit features an Enhancer Solution and PPT Buffer to ensure high-yield DNA extraction, even from plants with high levels of secondary products. Tested on over 100 plant and food samples, it provides comprehensive data and standard sample descriptions. Detailed, user-friendly instructions make this kit suitable for both research and regulatory applications, such as mandatory GMO labeling. Its versatility, robust performance, and ease of use make it an excellent choice for laboratories seeking consistent, high-quality results.
 
Research/Applicable Area
  1. PCR & Real-time PCR
  2. Southern Blotting
  3. SNP
  4. Gene Cloning
  5. Genotyping
  6. Pathogen research etc.

i-genomic Plant DNA Extraction Mini Kit

  • Item Quantity
    Buffer PG (Lysis Buffer) 30 ml × 1 ea
    Buffer PPT (Precipitation Buffer) 7 ml × 1 ea
    Buffer PB (Binding) 50 ml × 1 ea
    Buffer PWA (First-Wash) 40 ml × 1 ea
    Buffer PWB (Second Wash)(Concentration) 10 ml × 1 ea
    Buffer PE (Elution) 20 ml × 1 ea
    Enhancer Solution 0.5 ml × 1 tube
    Spin Column (Green color O-ring) 50 columns
    Collection Tube 100 tubes
    RNase A, lyophilized 3 mg × 1 tube
    Proteinase K, lyophilized 22 mg × 1 tube
    Manual 1 ea

  • Q: Is it possible to use gDNA prep for GMO test in vegetable beverages?

    A: It seems to be applicable if you separate the vegetable beverages separately. The appropriate protocol is considered to be applicable to Type E protocol (fruit). However, if applied directly in solution, the lysis itself may be diluted and not be prepped.

    Q: If you want to extract gDNA from lyophilized leaf, please recommend suitable product.

    A: Apply Type A protocol to i-genomic Plant. At this time, the lyophilized leaves are in the form of powder and do not require any homogenization, so use a small amount (5mg) of sample with little moisture content.

    Q: The gDNA prep is in progress in dry stem. Since the amount of supernatant is very small after PPT, is not it a product problem?

    A: Generally, in fresh stem tissues, 350 ~ 400ul of supernatant is formed after the PPT step, but dried stem has a low water content and about 150 ~ 200ul supernatant is formed. This is a feature of the sample and can be done according to the protocol.

    Q: I would like to extract DNA from sunflower seeds. I want to apply protocol G, but why is it less than other protocols?

    A: Gramineae Seed is weaker than other seeds and contains a lot of secondary products. Since the outer skin is thin and light, adding lysis buffer will float on top of the buffer. In addition, when the lysis process is carried out, the buffer is faded and blown.

    Q: Is not the protocol's binding capacity presented?

    A: The loading capacity, DNA binding capacity and elution volume are indicated on the "Notice Before Use" for each part.

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