The IQeasy™ Plus Plant RNA Extraction Mini Kit is a column spin-type product designed for efficient extraction of high-purity, high-yield total RNA from diverse plant specimens, including leaves, roots, stems, seeds, and legumes. It overcomes challenges associated with plant metabolites and inhibitors by eliminating the use of chaotrophic salts in the lysis process, which prevents co-precipitation and ensures inhibitor-free RNA extraction.
- Extracts total RNA from a wide range of plant samples, including seeds with high metabolite content.
- Achieves rapid RNA extraction (within 20 minutes) with a user-friendly protocol.
- Delivers high purity and yield of RNA, minimizing contamination.
- Removes genomic DNA (gDNA) effectively (up to 99%) without the need for DNase, using a two-column system.
- Avoids harsh chemicals (no chaotrophic salts used) and ensures inhibitors are not eluted with RNA.
- Provides reproducible results with specific protocols tailored to different plant tissues and species.
- Suitable for downstream applications such as microarrays and pathogen research.
This kit is trusted for its reliability, efficiency, and ability to extract high-quality RNA suitable for advanced research applications.
Research/Application Area
- Plant pathogen detection
- RT-PCR and real-time RT-PCR
- Microarray
- Northern Blotting
- Infectious Disease Research
- cDNA synthesis
IQeasy™ plus Plant RNA Extraction Kit
Contents Quantity Buffer RLE 25ml x 1 ea Buffer RB 20ml x 1 ea Buffer RLD 15ml x 1 ea Buffer RPB 15ml x 1 ea Buffer RW1(First Wash) 40ml x 1 ea Buffer RW2 (concentrate) (Second Wash) 10ml x 1 ea Buffer RE (Elution) 20ml x 1 ea Sieve Spin Columns 50 Columns gDNA Remover Spin Columns 50 Columns Binding Columns (Red O-ring) 50 Columns Collection Tubes (2.0 ml tubes) 50 tube Q: Sample volume 25 to 100mg is need to perform experiment according to protocol, however, what is the exact sample amounts need for experiment?
A: As a result of confirming the optimum use amount of soybean seeds, the optimum sample usage was confirmed at 50mg, which is 25 ~ 100mg because there may be slight difference depending on the sample.
Q: I have extract RNA from rice but no result came out. What is the reason for this problem?
A: "Protocol A" is the optimal protocol for seeds such as plant leaf or Solanceae, Cucurbitaceae. Fabaceae and Brassicaceae seeds containing Gramineae should proceed to "Protocol B".
Q: I have been using Plant RNA Extraction Kit from Q. I have been introduced to Intron's IQeasy plus Plant RNA product through my acquaintance. What are the characteristics of this product?
A: First, the iQeasy Plus Plant RNA Extraction Kit does not use any organic solvents such as phenol, but can be rapidly extracted using the column system, which does not require any additional enzyme treatment such as DNase I. In addition, Column Individual Packing (CAPS) is applied to prevent contamination.
Q: The protocol states that in the sample preparation stage, liquid nitrogen should be used to grind into a marsh bowl. Can I perform the experiment with the RLE buffer by chopping the raw sample without crushing the sample?
A: In addition to liquid nitrogen, it is also possible to use a pestle or homogenizer that can crush the sample.
Q: Is there spin type of kit for extracting RNA from fungi?
A: We do not provide spin type of kit for fungi RNA. IQeasy plus plant kit can be used after through preconditioning process.
