The Patho Gene-spin™ DNA/RNA Extraction Kit provides rapid and efficient extraction of DNA and RNA from a wide variety of pathogenic samples, including plasma, blood, serum, cell-free body fluids, and tissues infected by viruses or bacteria. Utilizing a low concentration of chaotropic salt and advanced lysis efficiency, this kit achieves optimal yield without the need for additional additives.
Stable extraction is ensured across multiple sample types, supporting both clinical diagnostics and research applications. The kit features a safer, non-phenol methodology and eliminates the need for ethanol precipitation, making it exceptionally user-friendly for laboratory professionals.
Ideal for use in disease diagnosis, forensic medicine, and other applications requiring high-quality nucleic acid extraction, the Patho Gene-spin™ Kit stands out as a reliable and effective solution for professionals seeking consistent results.
Research/Application area
- Pathogen Detection
- PCR or RT-PCR
- Quantitative PCR (qPCR, qRT-PCR)
- cDNA synthesis
- Infectious disease research etc.
Patho Gene-spin™ DNA/RNA Extraction Kit
Item Description Volume Lysis Buffer — 35 ml Binding
Buffer
— 35 ml Washing
Buffer A
— 30 ml Washing
Buffer B²
(concentrates)
Add 40 ml of EtOH before use 10 ml Elution
Buffer
— 20 ml Spin
Columns
Inserted into collection tubes
(2.0 ml tubes)
50 columns Instruction
Manual
— 1 sheet - Carefully handle the Lysis Buffer as it is composed of high concentration of chaotropic salt
Q: What is the yield of nucleic acid extraction from normal serum samples?
A: According to some literature reports, there are genome-derived substances from 500 to 1000 in serum or plasma samples derived from healthy individuals. In case of free-circulating DNA in plasma, it is known that it presents at the level of 1-100 ng/ml. This is a fairly large range, so it is practically impossible or insignificant to infer an average yield.
Q: Is it possible to extract viral nucleic acids from cell culture supernatant?
A: Yes, it can be used to extract viral nucleic acids from cell culture supernatant in case of following the protocol of this product.
Q: I want to know the yield of a small amount of DNA or RNA. Is there other way except for using A260 absorbance?
A: The Patho Gene-spin™ DNA/RNA Extraction Kit extracts whole nucleic acids present in a sample. This means that the product extracts not only nucleic acids from the pathogen in the sample but also nucleic acids from the host. Therefore, measuring the absorbance like A260 is not the preferred method. In addition, in the case of a small amount of nucleic acids less than 10 ng / μl, the range of error is large, so it is somewhat inaccurate to trust the measured value. In this case, it is preferable to perform Real-time PCR or Real-time RT-PCR or quantify fluorescent dyes binding to nucleic acids. Of course, since the detection of pathogens belongs to a qualitative study prior to quantification, it is more important to carry out fresh storage of the sample, rapid extraction and subsequent analysis, and it is not meaningful to measure the yield of extracted nucleic acids before use.
Q: Non-specific bands seem to be increased with the sensitivity of extraction. Is there a way to improve it?
A: Unlike serum and plasma samples, whole blood, buffy-coat and tissue emulsion contain large amounts of cells. The Patho Gene-spin™ DNA/RNA Extraction Kit maximizes the efficiency of nucleic acid extraction through effective dissolution of the sample. In result, overall yield is improved. In the case of samples with high cell contents, the amount of nucleic acids derived from the host is also large. Therefore, you can get clear amplification results if template usage is reduced to 1/10 level when non-specific amplification is observed during PCR analysis.
