• The Patho Gene-spin™ HS DNA/RNA Extraction Kit should be stored dry at room temperature (15–25°C) for up to 24 months without performance reduction.
• Freeze-dried proteinase K and carrier RNA must be stored at -20°C after opening, avoiding long-term room temperature storage.
• Reconstituted proteinase K and carrier RNA should also not be used for prolonged periods at room temperature.

Q: Little or no nucleic acid in the eluates?

A: If proteinase K and carrier RNA are degraded or not used properly, it may affect extraction performance. Check the conditions of use and storage of the components and use them according to the protocol. (2,4 pages)

If the concentration of pathogens in the sample is low, it is recommended to concentrate the sample volume to 150 µl using a micro concentrator. (Centricom-100 or Microcep 100)

- When processing cultured cells, the cell culture medium must be removed completely after collecting the cells.

- You maynot have followed the protocol properly or used an incorrect reagent.

Check the protocol and make sure the appropriate buffer is used for each extraction step. Cleaning buffer B is provided as concentrate. Make sure ethanol is added to cleaning buffer B before use.

- RNA is often broken down by RNase in the starting material. It is recommended to work quickly during sample preparation. If necessary, add RNase inhibitors to the sample.

- Using excessive samples greatly reduces purity and yield. After tissue sample homogenization and short centrifugation, transfer 150 µl supernatant to a new tube and add 300 µl dissolution buffer. Do not add homogenized pellets.

Q: Poor amplification?

A: If ethanol remains in the eluate, downstream application, such as the PCR reaction, may be inhibited. After cleaning buffer B, rotate the column at maximum speed for 1 minute to dry the patho gene-spin™ HS DNA/RNA Extraction kit membrane.