The RNA-spinTM Total RNA Extraction Kit is made to quickly and easily extract total RNA from cells and tissues. Without the use of chemical solvents or alcohol precipitation, it makes it possible to isolate high-yield, high-purity RNA in just 20 minutes, guaranteeing extremely repeatable outcomes.
This kit uses chaotropic salts and detergents, namely R-Buffer and beta-mercaptoethanol, to dissolve cells and tissues, denature nucleic acid-binding proteins, and block RNase activity without the use of phenol, in contrast to the easy-spinTM (DNA free) Total RNA Extraction Kit. This guarantees effective separation of RNA from proteins.
Furthermore, after EtOH is added, only RNA attaches to the silica-gel barrier, reducing contamination by DNA and proteins. The kit can swiftly and simply extract pure RNA without EtOH precipitation and functions safely without the use of hazardous organic solvents. The possibility of contamination is further decreased by using CAPS in individually packed columns.
Research/Application area
- Northern Blot Analysis
- cDNA synthesis
- RT-PCR
- Real-time RT-PCR
- Dot Blotting
- Primer Extension
- Infectious disease research, etc.
RNA-spin™ Total RNA Extraction Kit
Contents Quantity R-Buffer 20ml × 1 ea Washing Buffer A 40ml × 1 ea Washing Buffer B (Concentration) 10ml × 1 ea Elution Buffer 20ml × 1 ea Spin Columns (Orange color) 50 Columns Collection tubes 50 tubes Manual 1 ea Q: What is the differences between easy-spin kit and RNA-spin kit?
A: easy-spin kit is made by combining column type and solution type and RNA-spin kit is made by only column type.
Q: Is it possible to prepare RNA Prep using RNA-spin column after taking out aqueous layer with Trizol?
A: We sell easy-spin kit for RNA extraction. easy-spin kit has designed to customer taste. The lysis step is the same as the instruction, and RNA can be extracted with high purity and high yield using a column when RNA is recovered. Since the RNA-spin kit does not use an organic solvent, the recovery rate can not be guaranteed for column loading after lysis with Trizol. Therefore, we recommend using easy-spin, which has advantages of solution type and advantages of column type.
Q: RNA was extracted using RNA-spin and we used elution buffer provided from kit. Extracted RNA is use with metal but metal can change because of chemical and this led bad test results. If it possible, can I know components for elution buffer?
A: Based on our regulation, it is impossible to tell you the components. In case of using elution buffer, you can replace DEPC water for RNase free water. Please use RNase free water instead of using elution buffer.
