Introducing the Viral Gene-spin™ Viral DNA/RNA Extraction Kit, a state-of-the-art solution for the swift and efficient extraction of viral DNA/RNA from diverse clinical samples like serum, plasma, cell-free fluid, and stool. This OIE-listed product ensures extraction within a mere 20 minutes, delivering high yield and purity without the need for enzyme treatment. With a potent lysis buffer, it dissolves virus envelope and capsid proteins, facilitating easy elution and RNase inactivation.
The kit is non-phenol, requires no EtOH precipitation, and minimizes contamination by using CAPS. Safely extract pure viral genes for molecular diagnostic studies, minimizing the risk of contamination. This advanced kit not only streamlines the extraction process but also contributes to the reliability of results in molecular diagnostic studies, proving particularly beneficial in clinical diagnostics for identifying viral or bacterial infections in both humans and animals. The isolated and purified viral genes find utility in a range of applications, from viral diagnostics to cancer research, making the Viral Gene-spin™ Kit an indispensable asset in the realm of molecular biology.
Viral Gene-spin™ Viral DNA/RNA Extraction Kit
Content: 50 columns
Lysis Buffer: 30ml x ea
Binding Buffer: 40ml x ea
Washing Buffer A: 30ml x ea
Washing Buffer B(Concentration): 10ml x ea
Elution Buffer: 20ml x 1 ea
Spin Columns(Orange color): 50 columns
Collection Tubes: 50 tubes
Manual: 1 ea
Q: After infecting virus gene with cell line, experiment for checking cell condition has performed. Is it possible to only extract virus gene in order to use it for sequencing.
A: This kit not only extract virus but also genome(DNA/RNA). Therefore, it is recommended to sequester the target virus gene by amplifying and purifying the target virus gene by PCR.
Q: I am willing to extract only virus DNA, can lysis buffer lysed host’s nucleus membrane?
A: The nucleus membrane can also be lysed with the lysis buffer in the kit. Therefore, not only the virus gene but also the genome (gDNA, RNA) of the host cell can be extracted. This extracted DNA can be used as a sample for the detection of virus using PCR but it is impossible to isolate the nucleic acid of pure virus. The confirmation of virus infection can be determined by PCR or RT-PCR.
Q: I want to confirm infection of PCV from Pig’s tissue. Is it possible to extract viral DNA from tissue? If this works, please provide me protocol.
A: This kit is possible to extract viral gene from tissue. Generally, we need to prepare 10 to 25mg of sample, but we recommend 50mg for heart, brain muscle tissues. The protocol recommends to homogenize the Tissue and add PBS to make a total volume of 150ul. Then, move onto next step.
Q: We are currently developing a type B infection treatment. We are planning to extract HBV from HBV infected blood.A: We recommend Viral-gene spin Viral DNA/RNA Extraction kit. Viral-gene spin product has designed to easily extract viral genes from samples of various liquid components such as serum, plasma and cell culture within 20 minutes.
Q: When we apply the HBV infected blood sample for PCR using designed primer, however, no bands are shown while performing amplification. What is the solution?
A: This product is a product that can extract DNA or RNA virus from various liquids such as serum, plasma, etc., various liquid such as tissue emulsion liquid, feces suspension liquid, and cell culture liquid. You should first check the purity and yield of the extracted HBV sample and design the appropriate PCR condition accordingly. I think it is better to run PCR after confirming the specificity and sensitivity of the designed primer.