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Viral Gene-spin™ Viral DNA/RNA Extraction Kit
The Viral Gene-spin™ Viral DNA/RNA Extraction Kit efficiently extracts high-purity viral genetic material. This kit can isolate viral DNA and RNA from a variety of clinical samples, including serum, plasma, cell-free bodily fluids, stool, whole blood, tissue, swabs, urine, and cultured cells, within 20 minutes. It efficiently dissolves and denatures viral envelopes and capsid proteins, ensuring maximum nucleic acid production without the need for extra enzyme treatment.

For laboratory safety and convenience, the kit uses a non-phenol, non-ethanol precipitation process, making it both safe and easy to use for professionals. The use of CAPS in the columns reduces the chance of contamination while providing consistent and accurate results. The kit is appropriate for a wide range of clinical and scientific applications, including molecular diagnostics, viral detection, cancer research, and the identification of infections in humans and animals. 
 
Research/Application area
  1. Pathogen detection
  2. Microarray
  3. cDNA synthesis
  4. Reverse Transcriptase PCR (RT-PCR)
  5. Quantitative PCR (qPCR, qRT-PCR, etc.)

Viral Gene-spin™ Viral DNA/RNA Extraction Kit

  • Contents Quantity
    Lysis Buffer 30ml × 1 ea
    Binding Buffer 40ml × 1 ea
    Washing Buffer A 30ml × 1 ea
    Washing Buffer B (Concentration) 10ml × 1 ea
    Elution Buffer 20ml × 1 ea
    Spin Columns (Orange color) 50 Columns
    Collection Tubes 50 tubes
    Manual 1 ea

  • Q: After infecting virus gene with cell line, experiment for checking cell condition has performed. Is it possible to only extract virus gene in order to use it for sequencing.
    A: This kit not only extract virus but also genome(DNA/RNA). Therefore, it is recommended to sequester the target virus gene by amplifying and purifying the target virus gene by PCR.

     

    Q: I am willing to extract only virus DNA, can lysis buffer lysed host’s nucleus membrane?
    A: The nucleus membrane can also be lysed with the lysis buffer in the kit. Therefore, not only the virus gene but also the genome (gDNA, RNA) of the host cell can be extracted. This extracted DNA can be used as a sample for the detection of virus using PCR but it is impossible to isolate the nucleic acid of pure virus. The confirmation of virus infection can be determined by PCR or RT-PCR.

     

    Q: I want to confirm infection of PCV from Pig’s tissue. Is it possible to extract viral DNA from tissue? If this works, please provide me protocol.
    A: This kit is possible to extract viral gene from tissue. Generally, we need to prepare 10 to 25mg of sample, but we recommend 50mg for heart, brain muscle tissues. The protocol recommends to homogenize the Tissue and add PBS to make a total volume of 150ul. Then, move onto next step.

    Q: We are currently developing a type B infection treatment. We are planning to extract HBV from HBV infected blood.
    A: We recommend Viral-gene spin Viral DNA/RNA Extraction kit. Viral-gene spin product has designed to easily extract viral genes from samples of various liquid components such as serum, plasma and cell culture within 20 minutes.

     

    Q: When we apply the HBV infected blood sample for PCR using designed primer, however, no bands are shown while performing amplification. What is the solution?
    A: This product is a product that can extract DNA or RNA virus from various liquids such as serum, plasma, etc., various liquid such as tissue emulsion liquid, feces suspension liquid, and cell culture liquid. You should first check the purity and yield of the extracted HBV sample and design the appropriate PCR condition accordingly. I think it is better to run PCR after confirming the specificity and sensitivity of the designed primer.

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